A 25 weeks trial (from Jan. 7, 1971-July 1, 1971) was conducted in order to compare with the effect of different additives to the basic culture media KC (Knudson C), KM (Kano´s Medium) and MS (Murashige & Skoog) etc, on multiplication and preserving protocorm obtained in meristem culture (liquid culture) of Cymbidium Rasanna $quot;pinkie.$quot;
The results obtained are given below:
1. In case of liquid culture media the browning phenomenon was found to be directly related with the rate of the formation and multiplication of protocorms. The degree and timing of browning differed according to the additive substance used.
2. In case of KC liquid cultural media the addition of peptone (2gm/ℓ) hastened the browning. Similar trials with other cultivars are necessary.
3. Compared with KM and MS liquid cultural media, the browning is quicker in KC liquid cultural media resulting in making it unfit for long period culture (25weeks´). However as the growth in early period (5 weeks´) was best in KC, it seems to be useful cultural media for short period culture.
4. As the browning was delayed most in KM liquid cultural media it seems to be suitable for long period culture(25 weeks´).
5. As the browning occurs after 10-12 weeks in case of MS liquid cultural media it seems to be suitable for medium period cultivation.
6. The addition of peptone (2gm/ℓ) was effective in case of KM and MS liquid cultural media for the formation of protocorm and chlorophyll, but was harmful in case of KC liquid cultural media which had a different effect compared with KM and MS liquid cultural media.
7. In case of each liquid cultural media viz; KC, KM, and MS added with kinetin(0.1ppm) and 2·4-D (0.1ppm) the protocorm redivided after browning resulted in second multiplication.
The reason for redivision is not clear but it might be due to the interference between inorganic ingredients contained in the liquid cultural media. the substances formed during browning and the additive hormones.
Further trials seems to be necessary with other cultivars also for investigating this redivision phenomenon.
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